Deciphering RpoD-family sigma factors transcriptional regulatory network highlights their multifaceted roles under heat shock conditions in Salmonella Typhimurium [ChIP-exo]

We present genome-wide RpoD-family sigma factors (RpoD, RpoS, and RpoH) binding profiles and transcriptome profiles in Salmonella Typhimurium 14028s under both control (37°C) and sublethal heat shock conditions (42°C). These genome-scale experimental data show how three RpoD-family sigma factors simultaneously coordinate many types of cellular processes to orchestrate the overall response of S. Typhimurium to heat stress. [ChIP-mini] S. Typhimurium 14028s WT and rpoH-8myc tagged cell were grown to mid-exponential phage, cultured in M9 minimal 0.2% glucose with a biological replicate under both control (37°C) and sublethal heat shock conditions (42°C).