RELEASE-2021

Surface water microbe samples are collected at the same time and location as surface water cell count samples and surface water chemistry samples, which occurs once per month in wadeable streams (12 times per year), and every other month in lakes and rivers (6 times per year). In wadeable streams, samples are collected near the downstream S2 sensor location. In lakes and rivers, samples are collected near the buoy sensor set at 1 sampling depth in unstratified systems and up to 3 depths in stratified lakes. Surface water grab samples are filtered on 0.22 um Sterivex capsule filters, capped and flash-frozen in the field. The filters are stored and shipped frozen to an analytical facility for DNA sequence analysis using primer sets targeting the small subunit of the ribosomal RNA gene. Sequence data undergo QAQC and are processed bioinformatically using an in-house pipeline to identify unique sequence variants in the data and to taxonomically identify each sequence variant.

地表水微生物样本（surface water microbe samples）的采样时间与空间位置，与地表水细胞计数样本、地表水化学样本保持一致。其中，可涉水溪流（wadeable streams）的采样频次为每月1次（年采样12次），湖泊与河流的采样频次为每两月1次（年采样6次）。在可涉水溪流中，采样点设置于下游S2传感器（S2 sensor）附近；在湖泊与河流中，采样点设置于浮标传感器（buoy sensor）周边：在未分层水体中仅布设1个采样深度，在分层湖泊中最多布设3个采样深度。地表水瞬时水样（surface water grab samples）通过0.22 μm的Sterivex胶囊式滤膜（Sterivex capsule filters）过滤，随后密封封装并在野外完成快速冷冻。滤膜将以冷冻状态保存并运输至分析实验室，采用靶向核糖体RNA（ribosomal RNA）基因小亚基的引物组（primer sets）开展DNA序列分析。所得序列数据将先经过质量保证与质量控制（Quality Assurance and Quality Control, QAQC），随后通过自研生物信息学分析流程（in-house pipeline）进行处理，以识别数据中的独特序列变异体，并对每个序列变异体开展分类学鉴定。

搜集汇总

背景与挑战

背景概述

RELEASE-2021数据集提供了2014-2018年间湖泊、河流和溪流中表面水微生物群落的分类单元计数和相对丰度数据，基于16S和ITS标记基因测序并通过标准生物信息学流程处理。该数据集适用于研究水生微生物多样性、群落组成及其生态功能。