Evaluation of the APC activity during ongoing cyclin B1-GFP degradation in oocyte halves containing or devoid of chromosomes as well as in whole oocytes.

1time elapsing between the beginning and the end of degradation.2for each couplet of halves the data were normalized by setting the signal for chromosomal half at the beginning of degradation as 100.3the signal value at the end of degradation subtracted from signal value at the beginning of degradation gives the relative amount of cyclin B1-GFP which was degraded and thus defining the extent of degradation.4Extent of degradation divided by the duration of degradation gives the average efficiency of cyclin B1-GFP degradation.Data shown as Mean ± SD.The values marked with the same superscript differ significantly.*, #P¶P&P$P