EBF1 ChIP-seq after NICD1 induction
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE201527
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资源简介：
Quantitative EBF1 ChIP-seq analysis in EBF1wt- or EBF1E271A-expressing pro-B cells harboring doxycycline-inducible NICD1 expression after doxycycline treatment. Ebf1-/- c-kit+ lymphocyte progenitors isolated from fetal liver were first transduced with a doxycycline-inducible NICD1 expression system, selected for retroviral expression and then transduced with EBF1wt- or EBF1E271A-expressing retroviruses. Cells were sorted for retroviral expression, in vitro differentiated into pro-B cells and treated with doxycyline for 6 hours prior to EBF1 ChIP-seq analysis. Human B cell spike-in chromatin was used for spike-in normalization.
创建时间：
2022-10-01
