Human iPSC-derived renal collecting duct organoids model cystogenesis in ADPKD

In autosomal dominant polycystic kidney disease (ADPKD), renal cyst lesions predominantly arise from collecting ducts (CDs). We established expansion cultures of human iPS cell-derived ureteric bud tip cells, an embryonic precursor that gives rise to collecting ducts. Moreover, we succeeded in advancing the developmental stage of collecting duct organoids and showed that all collecting duct organoids derived from PKD1-/- human iPS cells spontaneously develop multiple cysts, clarifying the initiation mechanisms of cystogenesis.