Shinya Inoue (2011) CIL:11958, Chaetopterus sp. 1 Woods Hole MA, oocyte. CIL. Dataset

This is one of 4 grouped time lapse movies (CIL 11958-11962) revealing the responses of a meiotic oocyte spindle to various forms of micromanipulation by a glass microneedle. The needle is inserted, from below, through the egg cortex to the inner pole of the metaphase spindle. This movie, speeded up about 10-fold, shows the transient puckering in the cell membrane showing that the spindle pole is attached to the cortex. After the attachment has been broken, the cell surface rounds back up. The spindle is made visible by the use of polarizing optics.

本数据集包含四组连续时间推移电影之一（CIL 11958-11962），揭示了减数分裂卵母细胞纺锤体对玻璃微针进行各种形式微操作的响应。微针从卵细胞皮质下方插入，直至中期纺锤体的内极。该电影经过约十倍加速，展示了细胞膜上的瞬态凹陷，表明纺锤体极与皮质相连。在附着断裂后，细胞表面重新恢复圆形。通过偏振光学手段，纺锤体得以显现。