RNA-seq of mouse sorted alveolar macrophages

Sequencing of sorted alveolar macrophages (AMs) from WT mice, or from SPAM deleter (SPecific AM deleter; Siglecf-fl-DTR-fl x Epx-Cre) mice with adoptively transferred donor AMs, treated with PBS or 3 x interleukin-33 (IL33). Experiment 1 compares AMs from PBS treated and IL-33 treated WT mice and AMs from CD45.1 WT or IRF4 -/- adoptively transferred donor AMs into IL33 treated SPAM deleter animals. Experiment 2 compares WT or Batf3 -/- adoptively transferred AMs into IL33 treated SPAM deleter animals. Experiment 3 compares AMs from house dust mite (HDM) sensitized WT mice in a timecourse during HDM challenge. Experiment 4 compares AMs from Schistosoma Mansoni (SM) sensitized WT mice in a timecourse after SM challenge. Experiment 5 compares WT or Il4ra -/- adoptively transferred AMs into IL33 treated SPAM deleter animals.