Gene Expression Analysis of Laser Captired Purkinje Cells from the Post-Mortem Essential Tremor Cerebellum

RNA-seq evaluation of enriched Purkinje cells from the post-mortem human cerebellum. Purkinje cells were removed via laser capture microdissection and pooled for RNA-extraction and sequencing. 24 ET patients and 16 controls healthy age matched were compared. The Purkinje cell has shown extensive morphological changes in the ET cerebellum, but are less abundant than other cell types (Ex: Granule cells) by a factor of about 1:600. We sought to sequence enriched Purkinje cells from patients diagnosed with Essential Tremor and patients who are neurologicall clear.