Observed and deduced parameters of wild type and mutant cells.

The observed pseudopod data were derived from wild type (WT) cells starved for the indicated times, or from mutants starved for 5h. Indicated are the number of cells and pseudopodia analyzed. The data for λp are the means and SEM for pseudopodia; the data for φ and σφ are the means and SD for pseudopodia; the data for a and s are the means and SEM for cells. The data for the observed γobs were obtained by least square curve fitting to Eq. 4 of the dispersion of 20 cells during 15 minutes; the error indicates the 95% confidence level of the fit; θ = cos−1γ. The data for the MC simulated γMC were obtained by simulating 100,000 trajectories using the observed pseudopod parameters, and the corresponding value of γ was computed as described for the experimentally observed γ. Finally, γstep was calculated with Eq. 9, only using pseudopod parameters.