Short-read RNA-Seq experiment of major tissues from wild-type mice under fasting and fed treatments

Male C57BL/6 mice, sourced from the Jackson Laboratory, were acquired at 12 weeks of age. The mice were housed in groups of three per cage, with unrestricted access to water and a standard chow diet. For the experimental treatments involving fasting and fed states (n = 3 per group), the mice were either provided continuous access to food or subjected to a 24-hour fasting period before euthanasia at 10 am for tissue collection. The tissues harvested included muscle, liver, heart, lungs, both white and brown adipose tissues, spleen, and kidney. These tissues were immediately preserved in liquid nitrogen post-sacrifice to maintain their integrity for subsequent analyses.雄性C57BL/6小鼠购自杰克逊实验室（Jackson Laboratory），于12周龄时购入。实验小鼠以每笼3只的密度分组饲养，可自由饮水并采食标准实验饲料（standard chow diet）。针对禁食与进食两种实验状态（每组n=3），将小鼠分为两组：一组持续自由进食，另一组于安乐死前行24小时禁食；所有小鼠均于上午10点实施安乐死并采集组织样本。采集的组织涵盖肌肉、肝脏、心脏、肺脏、白色脂肪组织与棕色脂肪组织、脾脏及肾脏。样本经处死后立即置入液氮中保存，以维持组织完整性，供后续分析使用。