Data from: Genomic survey of the ectoparasitic mite Varroa destructor, a major pest of the honey bee Apis mellifera

These data represent V. destructor genomic annotations to be used for evolutionary comparison with other arthropods.
The ectoparasitic mite Varroa destructor has emerged as the primary pest of domestic honey bees (Apis mellifera).
Here we present an initial survey of the V. destructor genome carried out to advance our understanding of Varroa biology and to identify new avenues for mite control. This sequence survey provides immediate resources for molecular and population-genetic analyses of Varroa-Apis interactions and defines the challenges ahead for a comprehensive Varroa genome project.
Resources in this dataset:Resource Title: Data Dictionary - BLAST - annotated Varroa contigs. File Name: Data Dictionary genomic survey ectoparasitic mite varroa.csvResource Title: BLAST-annotated Varroa contigs. File Name: 1471-2164-11-602-s3.csvResource Description: Spreadsheet of Varroa destructor genomic contigs with significant similarity to the GenBank nr database by BLAST search. This is Additional File 3 from Cornman, et al. 2010.

We used BLASTX (for genomic contigs) and BLASTP (for ORFs of 90 residues or more) to identify genic sequences in the assembly. Sequences were initially searched against a database of five arthropod peptide predictions (Drosophila melanogaster, Anopheles gambiae, Pediculus humanus [a representative non-Dipteran insect], Daphnia pulex, and I. scapularis), with a minimum expectation of 10-8, and then secondarily against the eukaryotic Refseq database at the same expectation. In total, 13,031 contigs were identified with BLAST-detected similarity to database sequences.

本数据集收录了毁灭螨（Varroa destructor）的基因组注释信息，旨在与其它节肢动物进行进化比较研究。毁灭螨作为家养蜜蜂（Apis mellifera）的主要害虫，其寄生性蜱螨已逐渐成为研究焦点。本研究对毁灭螨基因组进行了初步调查，旨在深化对毁灭螨生物学的理解，并探寻控制螨虫的新途径。本序列调查为毁灭螨与蜜蜂之间分子和种群遗传学分析提供了即时资源，并明确了全面毁灭螨基因组项目所面临的挑战。数据集包含以下资源：

资源标题：数据字典 - BLAST - 毁灭螨连续片段注释。文件名：Data Dictionary genomic survey ectoparasitic mite varroa.csv

资源标题：BLAST-注释毁灭螨连续片段。文件名：1471-2164-11-602-s3.csv

资源描述：毁灭螨基因组连续片段的电子表格，通过BLAST搜索与GenBank nr数据库具有显著相似性。此为Cornman等人在2010年的补充文件3。

本研究采用BLASTX（针对基因组连续片段）和BLASTP（针对90个或以上残基的开放阅读框）识别组装中的基因序列。序列首先在五个节肢动物肽预测数据库（果蝇（Drosophila melanogaster）、按蚊（Anopheles gambiae）、人虱（Pediculus humanus [非双翅目昆虫的代表]、水蚤（Daphnia pulex）和蜱（I. scapularis））中进行搜索，最小期望值为10^-8，随后在相同期望值下对真核生物Refseq数据库进行二次搜索。共识别出13,031个连续片段，通过BLAST检测与数据库序列具有相似性。