Global and p53-dependent transcriptional response of fibroblasts to Klebsiella pneumoniae

Increasing evidence highlights the role of bacteria in the physiopathology of cancer. However, the underlying molecular mechanisms remains poorly understood. Several cancer-associated bacteria have been shown to produce toxins which modulate the tumor suppressor p53 and thereby interfere with the host defense against tumorigenesis. Here, we show that lipopolysaccharides from Klebsiella pneumoniae (Kp) strongly inhibit the host p53 pathway and impairs p53 transcriptional activity upon DNA damage and oncogenic stress, preventing its tumor suppressive function. Overall design: mRNA profiles (in triplicates) of BJ hTert immortalized fibroblasts treated with supernatant from Klebiella pneumoniae culture, doxorubicin and p53 shRNA