a-linolenic acid and long-chain n-3 polyunsaturated fatty acids modulate colitis operating with a different gene set

Adult Wistar rats were fed diet enriched with either a-linolenic acid (ALA) or fish oil for 8 (n=10) weeks. Colitis was induced in rats using 5% dextran sulfate sodium for 7 days. The rats were dissected and RNA was isolated from the colon tissues of rats, the samples from 6 rats were pooled and used for the microarray experiments. Global gene expression analysis was done in duplicates in each group and the results were compared to n-6 fatty acid group. a-linolenic acid (ALA) and fish oil rich in eicosapentaenoic acid and docosahexaenoic acid significantly attenuated the symptoms of colitis, compared to n-6 fatty acid group. Gene expression analysis has revealed that there is no common gene signature in the two groups.将成年Wistar大鼠分为两组，分别饲喂富含α-亚麻酸（a-linolenic acid, ALA）或鱼油的日粮，连续饲喂8周（每组n=10）。采用5%葡聚糖硫酸钠（dextran sulfate sodium）对大鼠进行结肠炎造模，造模时长为7天。处死后采集大鼠结肠组织并提取RNA，将6只大鼠的组织样本混合后用于基因芯片实验。每组设置双重复开展全基因表达分析，并将分析结果与n-6脂肪酸组进行对比。实验结果显示，相较于n-6脂肪酸组，富含α-亚麻酸（ALA）以及二十碳五烯酸（eicosapentaenoic acid, EPA）、二十二碳六烯酸（docosahexaenoic acid, DHA）的鱼油可显著缓解结肠炎症状。基因表达分析结果表明，两组间不存在共同的基因表达特征。