Expression data of J3T-1, J3T-2, J3T-1shA, J3T-2A glioma

We have previously established two sibling glioma subclones, J3T-1 and J3T-2, showing distinct invasive and angiogenic phenotypes. J3T-1, expressing high annexin A2, demonstrates robust angiogenesis and tumor invasion around neovasculature. J3T-2, expressing low annexin A2, demonstrates diffuse invasion along white matter tracts. Knockdown of annexin A2 in J3T-1 (J3T-1shA) resulted in diffuse invasion pattern, and overexpression of annexin A2 in J3T-2 (J3T-2A) showed prominent angiogenesis. We used microarrays to identify genes which promote the phenotypic transition regulated by annexin A2. Total RNA of J3T-1, J3T-2, J3T-1shA, and J3T2A were isolated, and each gene expression profile was investigated using Affymetrix Canine Genome 2.0 Array.