Data: Gene expression profile in peripheral B-lymphocytes and sera of individuals with fibromyalgia

Overview
Fibromyalgia (FM) is an idiopathic chronic disease characterized by widespread musculoskeletal pain, hyperalgesia and allodynia, often accompanied by fatigue, cognitive dysfunction and other symptoms. We investigated the gene expression profile in peripheral B-cells in FM patients and healthy matched control individuals.

Summary
The uploaded data consist of count table and sample information and can be used for gene expression analysis of patients and controls.

Generation of Data
A total of 100 ng of RNA from purified B-cells of each participant (26 patients and 23 controls) was used for sequencing following rRNA depletion and library preparation (TruSeq stranded mRNA library preparation kit, cat# 20020595, Illumina Inc.). Unique dual indexes (cat# 20022371, Illumina Inc.) were used according to the manufacturers’ protocol (#1000000040498). The quality of libraries was evaluated using the Fragment Analyzer from Advanced Analytical (AATI) using the DNF-910 kit and adapter-ligated fragments were quantified by qPCR using the Library quantification kit for Illumina (KAPA Biosystems) on a CFX384Touch (Bio-Rad) prior to cluster generation and sequencing. Sequencing was carried out on an Illumina NovaSeq 6000 instrument (NovaSeq control software v 1.7.0/ RTA v3.4.4) according to the manufacturers’ instructions. Demultiplexing and conversion to FASTQ format was performed using the bcl2fastq2 (v2.20.0.422) software, provided by Illumina. 

Data
Count Data: Samples were analyzed with the nf-core RNA sequencing pipeline release 1.4.2 (github.com/nf-core/rnaseq). In brief, the pipeline processes raw data from FastQ inputs, aligns the reads, generates counts relative to genes or transcripts.

Sample Data: Information regarding SampleName, Sample and Condition

{'Overview': '概述：纤维肌痛（FM）是一种病因未明的慢性疾病，其特征为广泛的肌肉骨骼疼痛、痛觉过敏和痛觉超敏，常伴有疲劳、认知功能障碍及其他症状。本研究调查了纤维肌痛患者和健康匹配对照个体的外周B细胞的基因表达谱。', 'Summary': '摘要：所上传的数据包括计数表和样本信息，可用于对患者和对照者的基因表达分析。', 'Generation of Data': '数据生成：每位参与者（26名患者和23名对照者）的纯化B细胞中总共提取了100 ng的RNA，用于测序，在去除rRNA和文库制备（Illumina Inc.的TruSeq单链mRNA文库制备试剂盒，猫号20020595）之后。根据制造商的协议（#1000000040498），使用了独特的双索引（Illumina Inc.的猫号20022371）。使用Advanced Analytical（AATI）的Fragment Analyzer和DNF-910试剂盒评估文库质量，在生成簇和测序之前，通过qPCR使用Illumina（KAPA Biosystems）的文库定量试剂盒在CFX384Touch（Bio-Rad）上对适配器连接的片段进行定量。根据制造商的说明，在Illumina NovaSeq 6000仪器（NovaSeq控制软件v 1.7.0/RTA v3.4.4）上进行测序。使用Illumina提供的bcl2fastq2（v2.20.0.422）软件执行去混池和转换为FASTQ格式。', 'Data': {'Count Data': '计数数据：样本使用nf-core RNA测序管道版本1.4.2（github.com/nf-core/rnaseq）进行分析。简而言之，该管道处理来自FastQ输入的原始数据，对读取进行比对，生成相对于基因或转录本的计数。', 'Sample Data': '样本数据：有关SampleName、Sample和Condition的信息'}}