Supporting data for "NanoGalaxy: Nanopore long-read sequencing data analysis in Galaxy"

Long-read sequencing can be applied to generate very long contigs and even completely assembled genomes at relatively low cost and with minimal sample preparation. As a result, long-read sequencing platforms are becoming more popular. In this respect, the Oxford Nanopore Technologies-based long-read sequencing nanopore platform is becoming a widely used tool with a broad range of applications and end-users. However, the need to explore and manipulate the specialised data generated by long-read sequencing platforms means that accompanying specialised bioinformatics platforms and tools are required in order to correctly process the long-read data generated. Importantly, such tools should additionally help democratise bioinformatics analysis by enabling easy access and ease-of-use solutions for researchers. The Galaxy platform provides a user-friendly interface to computational command-line-based tools, including their software dependencies and refined workflows. The interface enables researchers, who do not necessarily possess programming experience or extended computer skills, to perform powerful bioinformatics analysis, including the assembly and analysis of short- or long-read sequence data. The newly developed NanoGalaxy is a Galaxy-based toolkit for analysing long-read sequencing data, which is suitable for diverse applications, including <i>de novo</i> genome assembly from genomic, metagenomic and plasmid sequence reads. A range of best practice tools and workflows for long-read sequence genome assembly have been integrated into a NanoGalaxy platform in order to facilitate easy access and use of bioinformatics tools for researchers. Nanogalaxy is freely available at the European Galaxy server, which includes self-learning training material.