Richard Allen (University of Hawaii) (2011) CIL:20812, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

The CV membrane has collapsed into regularly spaced tubules oriented perpendicular to the microtubular ribbon in this ruptured cell. Some tubules and some nearby vesicles contain electron opaque material probably obtained from the mixture of the external medium and cytosolic contents as the CV expelled its content and medium had a chance to enter the CV by backflow. TEM taken on 8/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13115) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

该破裂细胞的细胞膜已塌陷成与微管带垂直排列的规则管状结构。部分管状结构和邻近的囊泡含有电子不透明物质，这些物质可能源自外部介质与细胞质内容的混合。在细胞质膜排出其内容物的同时，外部介质及细胞质内容有机会通过逆流进入细胞质膜。该透射电子显微镜图像由R. Allen于1996年8月8日使用Zeiss 10A型仪器在80kV电压下拍摄。负片放大倍数为31,500倍，刻度尺长度为0.2微米。该负片已打印到纸上，并通过Photoshop进行扫描。此数字化图像可用于定性分析。未经处理的、高分辨率的图像（CIL:13115）存档于图书馆，可供定量分析使用。该样本采用标准戊二醛固定，随后进行四氧化锇处理，用酒精脱水并浸入环氧树脂中。微切片制备厚度约为75纳米。更多详细信息请访问（http://www5.pbrc.hawaii.edu/allen/）。