Diurnal liver transcriptome in wt and nono-/- mice

The liver circadian transcriptome results from the combined action of a circadian clock and feeding/fasting rhythms. Since we found that (1) the number of NONO-containing speckles increases in response to feeding, (2) NONO interacts with RNA processing factors and (3) it binds to the introns of a large number of protein coding genes, we hypothesized that NONO may contribute to the daily rhythm of hepatic gene expression. To address whether NONO contributes to rhythmic gene expression in response to feeding/fasting, diurnal liver transcriptomes were assessed in nono-/- and wt littermate mice fed a normal diet during the night time (ZT12-24). We habituated mice to a 12:12 LD cycle with food available only at lights off (ZT12-24) for one week and in the second week collected mice every 2h throughout the 24h day. Both total and nuclear RNAs were sequenced after ribosomal RNA depletion Overall design: Livers were collected at 12 different time points during a 24h day (ZT0-ZT22). For each liver sample, total and nuclear RNA were extracted for a total of 48 samples. Each sample is a pool of 2 mice.