Relative activation of both T. castaneum SK receptors by 29 different peptides.

Each peptide was added to a well of a 96-well plate at a final concentration of 1 μM. The response of the sulfated SK from T. castaneum [sTrica-SK (5–13)] at this concentration was used as 100% value for each separate 96-well plate. Response of all other peptides was expressed relatively to this 100% response level. At least three biological repeats (each performed in duplicate) were used to quantify the relative response of each peptide at each receptor. These screens were performed in CHO-WTA11 cells.