Raw K562 experimental data and miRNA–Hippo interaction table for the imatinib–Hippo–miRNA CML study

Raw experimental data and miRNA–Hippo interaction table for “Time-resolved Hippo–YAP and microRNA responses to imatinib in CML K562 cells with in silico validation in CD34⁺ CML progenitors” Description of the data and file structure # Data for imatinib–Hippo–miRNA study in K562 cells Raw experimental data and interaction tables underlying the manuscript: Akbari S, et al. “Time-resolved Hippo–YAP and microRNA responses to imatinib in CML K562 cells with in silico validation in CD34⁺ CML progenitors” (PLOS ONE, in submission). ## Files - K562_MTT_viability_raw.xlsx Raw absorbance values for 48 h MTT viability assays at multiple imatinib concentrations. - K562_AOEB_apoptosis_counts_raw.xlsx Raw AO/EB cell counts for apoptosis quantification at 48 h (control vs imatinib). - K562_ROS_DCFHDA_raw.xlsx Raw DCFH-DA fluorescence readings for ROS measurements over time. - K562_gene_qPCR_Ct_raw.xlsx Raw Ct values for Hippo pathway genes and GAPDH reference. - K562_miRNA_qPCR_Ct_raw.xlsx Raw Ct values for Hippo-related microRNAs and U6 reference. - miRNA_targets_master.csv Curated miRNA–target interaction table used for the Hippo/miRNA network. - PhaseContrast_200x_control_48h_raw.tif - PhaseContrast_200x_imatinib_48h_raw.tif - AOEB_control_raw_48h.tif - AOEB_imatinib_raw_48h.tif Raw phase-contrast and AO/EB microscopy images corresponding to morphology and apoptosis panels. ## Code All R scripts for processing GSE12211/GSE14671 and reproducing the bioinformatics analyses are available at: https://github.com/soroushakbari/cml-hippo-imatinib-k562