Intensity of 0ΔNLS antiserum IgG binding of HSV-2 Proteins by MW: Area-Under-Curve Analysis.

a Protein molecular weight ranges analyzed for IgG binding intensity by calculating the area under the curve for each lane profile shown in S2 Fig.b Mean ± sem intensity of IgG binding in the indicated MW range in the HSV-2 lane of blots shown in Fig. 2. For each measurement, percentage of “summated signal for MW range” was calculated relative to the “summated signal for the entire HSV-2 lane.” The mean ± sem values were calculated from n = 5 Western blots incubated with serum of mice immunized with the HSV-2 0ΔNLS vaccine (S1 Fig.).Intensity of 0ΔNLS antiserum IgG binding of HSV-2 Proteins by MW: Area-Under-Curve Analysis.