five

Candidate list of Vpr-interacting partners in macrophages.

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Candidate list of Vpr-interacting partners in MDMs from two independent experiments. PBMCs obtained from healthy blood donors were purified using Ficoll-Hypaque gradient centrifugation. CD4+ T cells or monocytes were isolated from PBMCs via negative selection with CD14-positive enrichment cocktail (StemCell Technologies). Briefly, MDMs were generated by stimulating monocytes with 50 ng/mL recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D) for 7 days.
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2021-05-19
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