Acidity-mediated induction of FoxP3+ regulatory T cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE217536
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Regulatory T cells (Tregs) are known to maintain survival and suppressive function in the presence of high levels of extracellular lactic acid. However, the effect of lactic acid on Treg induction is not known. We therfore evaluated the effect of lactic acid on Treg induction and observed an increased induction of Tregs in the presence of lactic acid. This increase occurred in a glycolysis-independent, acidity-dependent manner. CD4+ T cells, purified from spleens of FoxP3RFP mice were stimulated (with anti-CD3 and anti-CD28) for 24 or 72h in the presence of TGFb in low glucose condition. During this 10mM lactic acid was added. Cells were sorted after the indicated timepoint based on expression of FoxP3RFP and cell pellets were snap frozen for RNA extraction and further analysis.
创建时间：
2023-03-25
