Counteraction of myocardial ferritin heavy chain deficiency by heme oxygenase-1

To investigate the functional significance of myocardial ferritin heavy chain (FtH) in a model of acute myocardial infarction (MI). FtH was deleted using FtH floxed mice and Sm-22 Cre mice on a C57/bl6 background At 3 hours following 45 minutes of ischemia induced via ligation of left ant. decsending coronary artery, ischemic regions were identified, dissected, and total RNA was isolated fand gene expression profiling analysis using data obtained from RNA-seq of 3 different animals. RNA Total RNA isolated from left ventricle under homeostatic (bseline conditions) served as negative ctr. (n=3/group) Overall design: Comparative gene expression profiling analysis of RNA-seq data for widltype and FtH KO myocardium at baseline and 3 hours post ischemia