Inhibition of H3K9me2-specific histone methyltransferase, G9a/GLP, reveals transcripts regulated by the establishment of H3K9me2 in hematopoietic stem and progenitor cells

We have performed RNA-seq from DMSO control treated, G9a/GLP inhibitor (UNC0638) treated, and DNA-demethylating agent (Decitabine) treated CD34+ hematopoietic stem and progenitor cells. This experiment was performed to identify transcripts that were uniquely affected by the loss of H3K9me2 in hematopoietic stem and progenitor cells. Comparative gene expression profiling analysis of RNA-seq data for CD34+ hematopoietic stem and progenitor cells treated with DMSO, UNC0638, or Decitabine