Investigating the effects of estrogen in the transcriptional regulation of SOX2 in breast cancer cells.. Investigating the effects of estrogen in the transcriptional regulation of SOX2 in breast cancer cells.

We have evaluated the effects of estrogen over SOX2 expression by maintaining MCF-7 under three distinct conditions: estrogen deprivation for a year (LT -E2 MCF-7), treating MCF-7 cells with estrogen for 48 hours (48hr +E2 MCF-7), and maintaining cells under estrogenic conditions for a year (LT +E2 MCF-7). Overall design: LT -E2 MCF-7 cells were maintained in estrogen-deprived conditions for a year; 48hr +E2 MCF-7 cells were treated with 10nM b-estradiol for 48 hours; LT -E2 MCF-7 cells were grown under 10nM b-estradiol for a year. Cells were submitted for ATAC-seq and ribo-depleted total RNA-seq in three replicates each.