The secretome of irradiated peripheral blood mononuclear cells attenuates activation of mast cells and basophils. The secretome of irradiated peripheral blood mononuclear cells attenuates activation of mast cells and basophils

Effect of PBMCsec on the transcriptional profile of dermal mast cells were investigated in a dataset of PBMCsec-treated female Balb/c mice dorsal skin. Overall design: Single cell RNA-sequencing gene expression comparison of mouse dorsal skin subcutaneously injected with PBMCsec or vehicle control. Single-cell suspension from four samples per condition were prepared using MACS Miltenyi Whole Skin Dissociation Kit (Miltenyi). Libraries were generated using Chromium Next GEM Single Cell 3´GEM, Library & Gel Bead Kit v3.1, Chromium Nexxt GEM Chip G Single Cell Kit and Single Index Kit T Set A (all 19x Genomics, Pleasanton, CA, USA).