Closing the genome of unculturable cable bacteria using a combined metagenomic assembly of long and short sequencing reads

A novel approach for obtaining closed genomes of unculturable bacteria, using the enigmatic cable bacteria as a case study. The approach involves the development of a clonal enrichment culture and metagenomic hybrid sequencing. As a result, the first closed genome of the Ca. Electrothrix genus was achieved (Ca. Electrothrix scaldis GW3-3).