Levels of sema1a correlate with performance in yeast behavioral assays.

The above set of data represents a compiled summary of results obtained from four replicate experiments in which control-fed vs. sema1a knockdown (KD) animals were tested in a yeast odorant attractant assay. The total number of animals (n) indicates the number of individuals that were assessed in these assays. The number of individuals (# Animals) that were attracted (left; animals that touched the yeast pellet and received a score of 1) or not attracted (right; animals that did not touch the yeast pellet and received a score of 0) under each condition (Control or KD) are indicated, and the percentages of total animals are reported after the raw numbers. The levels of sema1a were assessed in the brains and antennae of animals attracted (left) or not attracted (right) to the yeast. The raw number/percentage of # animals with Normal (wildtype sema1a), Null (no detectable sema1a transcript), or Moderate (reduced but not wildtype sema1a) levels are indicated. Loss of sema1a expression correlated well with a lack of attraction to the yeast.