Ultrastructural analysis of Glu-ArxKO and Dox+ IndGlu-ArxKO pancreata by electron microscopy.

Classical electron microscopy examination was combined to insulin detection using immuno-gold labeling to analyze islets of 4 month-old WT (A–B), 5 month-old Glu-ArxKO (C–D) and 11mDox+ IndGlu-ArxKO (E–F) animals. In mutant animals, all cells displaying a β-cell ultrastructure expressed insulin (inlets in D–F compared to B). Similarly, all cells labeled with insulin exhibited a typical β-cell ultrastructure (n = 3, 200 photographs analyzed per sample).