Adenovirus transducion of CV1.eSPR on in vitro differentiated human primary macrophages

Adenovirus transducion of CV1.eSPR on in vitro differentiated human primary macrophages Overall design: Buffy coat was isolated from leukapheresis cones by Ficoll-Paque centrifugation. Monocytes were enriched from human PBMC by CD14 beads positive selection (Miltenyi Biotec) and cultured in IMDM supplanted with 10% human AB serum (omega scientific, Cat# HS-20) for 6 days. Cells were replated. On day7, cells were transduced with MOI=1000 CV1.eSPR carrying adenovirus. On day9, RNA were extracted.