File S1 - Progressive Degradation of Crude Oil n-Alkanes Coupled to Methane Production under Mesophilic and Thermophilic Conditions

Figure S1, Time course of methane production of the pre-enrichment cultures incubated at 35°C (A) and 55°C (B). The square and rhombus represent the pre-enichment cultures incubated at 35°C, The triangles represent the pre-enrichment cultures incubated at 55°C. The arrows indicates the sampling points for transfer incubation of the pre-enrichment cultures. Figure S2, Rarefaction curves constructed from archaeal and bacterial 16S rRNA gene libraries based on OTU cutoff of equal or higher 97%. Figure S3, Phylogenetic tree based on archaeal 16S rRNA gene sequences from representative clones of each OTUs, related type strains and environmental clones using neighbor-joining analysis of 779-nt alignment. Representative clones from the mesophilic consortium are indicated in red, followed by in silico T-RFs and clone numbers, and the representative clones from the thermophilic consortium are indicated in blue, followed by in silico T-RFs and clone numbers. Figure S4, Phylogenetic tree based on bacterial 16S rRNA gene sequences from representative clones of each OTUs and related strains and environmental clones using neighbor-joining analysis of 698-nt alignment. Representative clones from the mesophilic consortium are indicated in red, followed by in silico T-RFs and clone numbers, and the representative clones from the thermophilic consortium are indicated in blue, followed by in silico T-RFs and clone numbers, the OTUs less than 3 clones in the mesophilic consortium and 2 in the thermophilic consortium were not shown in the phylogenetic tree. Methanothermobacter crinale (EF554596) was used as outgroup. The scale bar represents 2% sequence divergence. Table S1, Phylogenetic affiliation of archaeal 16S rRNA genes and corresponding theoretical T-RFs retrieved from methanogenic oil-degrading consortia at 35 and 55°C, respectively. Table S2, Phylogenetic affiliation of bacterial 16S rRNA genes and corresponding theoretical T-RFs retrieved from mesophilic methanogenic oil-degrading consortium. Table S3, Phylogenetic affiliation of bacterial 16S rRNA gene and corresponding theoretical T-RFs retrieved from thermophilic methanogenic oil-degrading consortium. (DOCX)