SMARTer single cell total RNA sequencing [FACS sorted cells]. SMARTer single cell total RNA sequencing [FACS sorted cells]
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA540673
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Before sorting, cells were washed twice in 1X PBS buffer (DPBS without calcium chloride and magnesium chloride; Sigma Aldrich, D8537) and labelled with 7-AAD (BD Pharmingen, 51-68981E) for live/dead differentiation and FITC-conjugated antibody [anti-CD47 (BD Pharmingen, 556045) for A375 and anti-CD81 (BD Pharmingen, 551108) for Jurkat]. Overall design: single cell total RNA-seq data was generated for FACS sorted A375 and Jurkat cells
创建时间：
2019-05-01
