Glucocorticoid receptor profiling in IMR90,K562 and U2OS (ChIP-seq). Glucocorticoid receptor profiling in IMR90,K562 and U2OS (ChIP-seq)

To identify the sequences responsible for recruitment of Glucocorticoid receptor (GR) to individual loci, we performed ChIP-seq and ChIP-exo that combines chromatin immunoprecipitation with an exonuclease digestion step. We performed these experiments in three cell lines : IMR90 (ATTC:CCL-186), U2OS osteosarcoma cell lines, K562 (ATCC:CCL243), upon glucocorticoid treatment.