Multiple sequence alignment for observed and previously reported [17], [18] amino acid sequence variations in prestin.

Amino acid alignments of prestin orthologs from various species are shown using H. sapiens as the anchor sequence. Amino acid (aa) positions 67, 150, and 663 indicated in the top row of columns 3–5 refer to the amino acid positions in the human prestin amino acid sequence. Reference sequences used are derived from the NCBI protein database and aligned using the NCBI HomoloGene multiple sequence alignment tool (http://www.ncbi.nlm.nih.gov).