Single-cell RNA sequencing of Cd45+ adventitial cells from mice at steady state or treated with AngiotensinII

Here we show, that arterial macrophages derive predominantly from YS EMPs, which give rise to a transcriptionally distinct cluster of macrophages with mainly homeostatic and anti-inflammatory properties in steady state as well as in response to AngII inflammation. In adult mice, these EMP-derived macrophages persist for long periods of time, but decreas in absolute numbers in senescence without compensation by BM-derived progenitors. EMP-derived macrophages expressed high levels of homeostatic genes such as Lyve-1, which links them to physiological functions in arteries. Overall design: Examination of adventitial immune cells in RankCreRosa26eYFP in two conditions (steady state and AnII-induced inflammation)