Transcription Factors Dlx1/2 Are Required for Making All Interneurons in the Olfactory Bulb [Pax6]

Purpose: To assess changes in gene expression profiles from the E16.5 wild type littermate controls cortex and Pax6 sey/sey mutant mice cortex. Methods: Total RNA was isolated and sequenced using an Illumina Hi-seq 2500. Raw data was analyzed using TopHat. Genes were considered significantly changed which perform P value <= 0.05. Results: Compared to controls, 173 genes were significantly down regulated, and 245 genes were up regulated in the RNA expression level of the mutant cortex. Cortex RNA profiles of E16.5 Wild type mouse brain and Pax6 sey/sey mutant mice brain.研究目的：评估E16.5胚胎期野生型同窝对照小鼠大脑皮层与Pax6 sey/sey突变型小鼠大脑皮层的基因表达谱变化。实验方法：提取总RNA并采用Illumina Hi-seq 2500进行测序；原始数据通过TopHat进行分析；将P值≤0.05的基因认定为存在显著表达差异的基因。实验结果：与对照组相比，突变型小鼠大脑皮层的RNA表达谱中，共有173个基因显著下调，245个基因显著上调。本数据集涵盖E16.5胚胎期野生型小鼠大脑及Pax6 sey/sey突变型小鼠大脑的皮层RNA表达谱。