Neuronal migration depends on blood flow in the adult mammalian brain

In animal tissues, several cell types migrate along blood vessels, raising the possibility that blood flow influences cell migration. Here, we show that blood flow promotes the migration of new olfactory-bulb neurons in the adult brain. Neuronal migration is facilitated by blood flow, leading to the accumulation of new neurons near blood vessels with abundant blood flow. Blood flow inhibition attenuates blood vessel-guided neuronal migration, suggesting that blood contains factors beneficial to neuronal migration. We found that ghrelin, which is increased in the blood by hunger, directly influences neuronal migration. Ghrelin signaling promotes somal translocation by activating actin cytoskeleton contraction at the rear of the cell soma. New neurons mature in the olfactory bulb and contribute to the olfactory function for sensing odorants from food. Finally, we show that neuronal migration is increased by calorie restriction and that ghrelin signaling is involved in the process. This st..., , # Data from: Neuronal migration depends on blood flow in the adult mammalian brain

Dataset DOI: [10.5061/dryad.dncjsxmcb](https://doi.org/10.5061/dryad.dncjsxmcb)

## Description of the data and file structure

We performed in vitro and in vivo experiments by immunohistochemistry and fluorescent live imaging.

### Files and variables

#### File: p_value.csv

Description: p-value from all data

#### File: Figure5-figure_supplement_4.csv

Description: Proportion of NeuN+/Dcx- cells among total BrdU+ cells in the OB in the ad libitum (AL) and 70% calorie restriction (CR) groups

#### File: Figure5-figure_supplement_3B.csv

Description: Proportion of Ghsr1a-KD cells in the OB in the Sham and bilateral carotid artery stenosis (BCAS) groups analyzed by immunohistochemistry in 6 to 12-week-old adult mice

#### File: Figure5-figure_supplement_2.csv

Description: Quantification of Ki67+ cells in the control/ghsr1a-KD groups in vivo

#### File: Figure5-figure_supplement_1.csv

Description: Analy...,