Establishment and evaluation of a multiplexPCR amplification-based sequencing methodfor respiratory virus type A: Supplementary data
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https://figshare.com/articles/dataset/Establishment_and_evaluation_of_a_multiplexPCR_amplification-based_sequencing_methodfor_respiratory_virus_type_A_Supplementary_data/24146973
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资源简介：
Aim: This study aimed to establish a simple whole-genome sequencing method for respiratory virus type
A (RSVA) used in genomic epidemiological studies. Methods: Multiple primers were designed to amplify
overlapping amplicons specific to RSVA. The amplicons were sequenced using Illumina and Nanopore
sequencing platforms and the sequencing performances of the two platforms were also compared.
Results: The study shows that the developed method could recover almost full genomes of RSVA when
Ct values were less than 33 for clinical samples. Conclusion: In this study, a whole-genome sequencing
method was developed for RSVA based on multiplex PCR.
创建时间：
2023-09-15
