RNA Sequencing of Intratumoral Immune Populations in Pancreatic Ductal Adenocarcinoma Following Radiotherapy and Interleukin 12 Treatment. RNA Sequencing of Intratumoral Immune Populations in Pancreatic Ductal Adenocarcinoma Following Radiotherapy and Interleukin 12 Treatment

We report differential gene expression in inflammatory monocyte (IM), tumor-associated macrophage (TAM), and CD8 T cell populations following stereotactic body radiation (SBRT), intratumoral interleukin 12 microsphere (IL-12 MS) delivery, and SBRT/IL-12 MS combination. Immune cell populations were sorted from orthotopic murine pancreatic ductal adenocarcinoma (PDA) tumors prior to RNA sequencing. Overall design: Orthotopic murine PDA tumors (KCKO cell line) were treated with SBRT, IL-12 MS, or SBRT/IL-12 MS combination (duplicate/triplicate); IM, TAM, and CD8 cells were sorted from tumor lysates using a FACSAria II platform; cDNA libraries were constructed using the TruSeq RNA Sample Preparation Kit V2 and sequencing was performed on a HiSeq 2500 platform.