Molecular identification and phylogenetic analysis of Aphelenchoides bicaudatus based on 28S rRNA D2-D3 expansion segments

This project presents the molecular identification of a fungivorous nematode isolate, morphologically identified as Aphelenchoides bicaudatus. The D2-D3 expansion segments of the 28S ribosomal RNA gene were amplified using the universal nematode primers D2A (5'-acaagtaccgtgagggaaagttg-3') and D3B (5'-tcggaaggaaccagctacta-3')