Uptake assays of endocytic markers by Toca-1 and N-WASP expressing cells.

Cells were transfected with Toca-1 and N-WASP and left to express the proteins for 24–36 hr. Markers for uptake pathways were then added and uptake monitored for 10 min for transferrin and 30 min for dextran and cholera toxin B (For images see Fig. S4). The cells were then washed, fixed and examined by confocal microscopy. Quantification of the % uptake of different markers in cells transfected with Toca-1-N-WASP compared with control cells as described in Material and methods section. The % uptake is an average +/− S. D., with n = 10, from 3 experiments.