RNA sequencing of HAP1 WT and MED13 KO clones

Purpose: The aim of the experiment was to eludicate differentialy expressed genes (DEGs) upon treatment with alkylating agent MMS between WT and MED13 deficient HAP1 cells. Results: After bioinformatic processing, at =2-fold change and an FDR=0.1, 446 DEGs were identified in MED13 KO cells when compared to WT. Upon the MMS treatment 394 DEGs were identified in MED13 KO cells, of which 229 were common with untreated MED13 KO cells. Conclusions: Common DEGs represented genes directly regulated by MED13. Overall design: Each genotype were analysed in 3 biological replicates for both MMS treated and untreated conditions. Control sample were represented by HAP1 WT untreated replicates.