Mauricio Valerio-Santiago, Fernando Monje-Casas (2011) CIL:13882, Saccharomyces cerevisiae. CIL. Dataset

Tem1 normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase (this image and CIL# 13885). This image shows SPB localization of eGFP-Tem1 (eGFP, green) in metaphase cells. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. Nuclear morphology was assessed by DAPI (blue). A differential interference contrast (DIC) image is also shown (gray). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 1A, top panels, in J Cell Biol. (2011) 192: 599-614. Other images in Fig 1 include CIL #13882, 13883, 13884, 13885, 13886, 13887.

Tem1通常优先定位于纺锤体极体（SPB）上，该极体在分裂中期进入子细胞（如图所示及CIL# 13885）。本图展示了处于有丝分裂中期的细胞中eGFP-Tem1（eGFP，绿色）的SPB定位。tem1Δ::GAL-UPL-TEM1细胞通过CEN质粒表达eGFP-TEM1，并在2%蔗糖/2%半乳糖培养基中培养，随后转移至2%葡萄糖培养基中进行图像捕获。通过DAPI（蓝色）对核形态进行了评估。同时展示了一幅差异干涉对比（DIC）图像（灰色）。tem1Δ::GAL1-UPL-TEM1菌株允许对Tem1进行快速、条件性耗竭。UPL（代表泛素-脯氨酸-LacI）作为一种不稳定性模块，可促使附加蛋白的快速降解。图像为J Cell Biol. (2011) 192: 599-614中的图1A，顶部面板。图1中的其他图像包括CIL #13882, 13883, 13884, 13885, 13886, 13887。