Additional file 6: of The piRNA pathway responds to environmental signals to establish intergenerational adaptation to stress

Figure S4. Limited duration of temperature-dependent intergenerational fitness effects. (A) Intergenerational Competition on E. coli at 25 °C between animals derived from (left) P0 animals grown at 25 °C marked with GFP and unmarked P0 animals grown at 20 °C; (right) unmarked P0 animals grown at 25 °C and P0 animals grown at 20 °C marked with GFP. Y-axis shows the percentage of unmarked animals grown after 2–3 generations (1 transfer, 5 biological replicates per condition), corrected for the effect of GFP at 25 °C. (B) Intergenerational competition on E. coli at 20 °C between animals derived from (left) unmarked P0 animals grown on E. coli at 20 °C and GFP-marked P0 animals grown at 25 °C (right) GFP-marked P0 animals grown at 20 °C and unmarked P0 animals grown at 25 °C. Y-axis shows the percentage of unmarked animals after 2–3 generations (1 transfer, 5 biological replicates per condition), corrected for the effect of GFP at 25 °C. (C) Multi-generational competition on E. coli at 25 °C between animals derived from (left) unmarked P0 animals grown at 20 °C and SNP-marked parents grown at 25 °C (right) SNP-marked P0 animals grown at 20 °C and unmarked parents grown at 25 °C. Y-axis shows the percentage of unmarked animals after 3–4 generations (2 transfers, 5 biological replicates per conditions), corrected for the effect of the SNP at 25 °C. (D) Multi-generational competition on E. coli at 20 °C between animals derived from (left) unmarked P0 animals grown on E. coli at 25 °C and SNP-marked P0 animals grown on S. marcescens at 25 °C (right) SNP-marked P0 animals grown on E. coli at 25 °C and unmarked P0 animals grown on S. marcescens at 25C. Y-axis shows the percentage of unmarked animals after 3–4 generations (2 transfers, 5 biological replicates per conditions), corrected for the effect of the SNP at 25 °C. (XLSX 44 kb)