Gene expression analysis of 120 hpf zebrafish livers following 24 hour iAs exposure. Gene expression analysis of 120 hpf zebrafish livers following 24 hour iAs exposure

This study was carried out to compare the changes in the gene expression in the liver of 5 day post fertilization zebrafish larvae in response to 24 hour exposure to 1.0 and 1.5mM iAs and to compare this to changes in gene expression as a result of overexpress as3mt in hepatocytes. CL1 CAAX 1.5 = WT larvae exposed to 1.5 mM iAs; CL2 CAAX 1 = WT larvae exposed to 1.5 mM iAs Overall design: The samples in this series include only livers from 120 hpf zebrafish larvae that were exposed to 1 and 1.5 mM of inoragnic arsenic (iAs) from 96 - 120 hpf. This study was carried out by comparing RNA extracted from 120 hpf zebrafish livers from six groups: untreated or 24-hour exposure to 1 mM or 1.5 mM iAs in CAAX-EGFP positive larvae and CAAX-EGFP positive and as3mt overexpressing larvae. To generate these samples ,(tg:(fab10a:as3mt, cry:dsRed) were outcrossed to tg(fabp10a:CAAX-EGFP) and the larvae that only had the CAAX-EGFP expression in the liver and cry:dsRed in the eye were collected. RNA from 12 livers per group collected from 5 clutches (biological replicates) per group were extracted using Trizol/Chlorofom, PolyA selected and sequenced on Illumina NextSeq550. The control samples are contained in series GSE156412.