John Murray (University of Pennsylvania), Ke Hu (Indiana University, Bloomington) (2011) CIL:10541, Toxoplasma gondii RH. CIL. Dataset

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. This is a maximum intensity projection from z slices 3-6 from deconvolved data stack: CIL# 10539. Maximum intensity projection from z slices 10-13: CIL# 10540. Corresponding raw data stack: CIL# 10538 and raw data stack with DIC: CIL# 10543.

Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

本研究采用单层人皮肤成纤维细胞，感染表达YFP标记的TgIMC1（内膜复合体支架蛋白）和mCherry标记的人α1微管蛋白的弓形虫 gondii。图像采集于Olympus IX70倒置显微镜，使用API DeltaVision系统。实验目的：Olympus 60X水浸式物镜，1.2 NA数值。滤光片：mCherry，激发波长555-592nm，发射波长600-665nm；YFP，激发波长490-510nm，发射波长525-595nm。图像捕捉采用CoolSnapHQ/ICX285相机。此为从去卷积数据栈的z切片3-6的最大强度投影：CIL# 10539。z切片10-13的最大强度投影：CIL# 10540。对应的原始数据栈：CIL# 10538，以及带有DIC的原始数据栈：CIL# 10543。此图像与PMID: 16518471发表的图像相似，详见原文获取其他实验细节。