Effect of miR-27a-5p on SGBS adipogenesis

We have recently confirmed miR-27a-3p as a crucial regulator of human adipogenesis (Wu H, Pula T, Tews D, Amri E-Z, Debatin K-M, Wabitsch M, Fischer-Posovszky P, Roos J. microRNA-27a-3p but Not -5p Is a Crucial Mediator of Human Adipogenesis. Cells. 2021; 10(11):3205. https://doi.org/10.3390/cells10113205 ). MiR-27a-5p did not impair human adipogenesis. However, since several publications state that miR-27a ist also a crucial regulator of UCP1, we were interested if miR-27a-3p or miR-27a-5p regulatas UCP1 and other thermogenesis related genes. We found a strong regulation of UCP1 with functional relevance for the cellular metabolism by miR-27a-5p.To asesse the mRNA gene expression pattern, mRNA sequencing was performed. For mRNA Sequencing RNA samples from SGBS cells transfected two days prior adipogenic induction with either 20 nM Syn-hsa-miR-27a-5p or non-target control were harvested on day 8 of adipogenesis and sent for sequencing to Eurofins Genomics (Ebersberg, Germany). Further analysis was performed by the Core Unit Bioinformatics and Datamanagement of Ulm University.