CE7R+ T Cells are Detected in the Peritoneal Wash of Intraperitoneal L1-CAM+ Tumor Bearing Mice.
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NSG mice recived i.p. injection of 10e5 ffLuc+ OVCAR-3 tumor cells on day 0, and were treated with asingle dose of 2.5 x 10e7 CE7R-transduced T cells i.p. (day 7). Flow cytometric detection of human T cells in the peripheral blood at day 22 (A) and in the peritoneal wash upon euthanasia (B) of representative mice (Mouse 1 and Mouse 2) at either day 22 or day 30 as indicated. Percentages of human CD45+ cells (A, B), or human CD45-gated cells that were stained with biotinylated anti-Fcγ followed by SA-PE to detect the CAR (B), are indicated in each histogram. C, Quantitative bioluminescence imaging of tumor growth in Mouse 1 (top) and Mouse 2 (bottom) over time. Mean flux levels of luciferase activity were measured. Dashed lines represent day of T cell treatment. (PDF)
