PEDV N interactiome _Label-Free.xlsx

Porcine epidemic
diarrhea virus (PEDV) is the agent of PED which causes numerous economic losses
for the swine industry each year. Till
now, no effective vaccine or drugs are available to contain this disease. As a
result, it is critical urgent to elucidate the PEDV interactome. The nucleocapsid
(N) of PEDV plays a key role in viral replication. In this study, the N gene was cloned into pEGFP-C1 and the recombinant plasmid pEGFP-N was prepared. In order to obtain the interactome of N protein, the plasmids pEGFP-N and
pEGFP-C1 were transfected in 293T and the cellular binding partners were pulled
down using the GFP-trap. These proteins were identified by label-free
MS. The results showed that approximately 1200 cellular proteins were initially
identified and quantified, which represented both specific and nonspecific interactions. Under the criteria of more
than 2 unique peptides, a false discovery rate (FDR) ≤ 1%and a p value <0.05
for the t-test analysis, 147cellular potential proteins were identified (as listed in the table).